How to reconstitute NAD+

The steps for mixing a NAD+ vial with bacteriostatic water, and the units every dose comes to after the mix is done.

Sizes of vials sold
500, 1,000 mg
For 500 mg, water gives 100 mg/mL
5 mL
bacteriostatic water after opening
28 days
How to reconstitute NAD+: water added to the vial wall

Mixing NAD+ step by step

  1. Using an alcohol swab, wipe both rubber stoppers, on the peptide vial and on the bacteriostatic water, after washing your hands.
  2. Draw the amount of water you chose into the syringe. A 500 mg vial with 5 mL gives 100 mg per mL.
  3. Insert the needle at a slight angle through the stopper. Let the water flow slowly along the vial's inner wall.
  4. Gently roll the vial between your hands until the powder disappears. Don't shake it.
  5. Note the mix date on the vial, then store it as your product or prescription directs.
  6. First calculate how many units your dose needs; then draw to that line.

Reconstitution chart for NAD+

VialWater addedStrengthUnits for a sample dose
500 mg2 mL250 mg/mLunder 1 unit for 1 mg
500 mg3 mL166.667 mg/mLunder 1 unit for 1 mg
500 mg5 mL100 mg/mL1 unit for 1 mg
500 mg10 mL50 mg/mL2 units for 1 mg
1,000 mg2 mL500 mg/mLunder 1 unit for 1 mg
1,000 mg3 mL333.333 mg/mLunder 1 unit for 1 mg
1,000 mg5 mL200 mg/mLunder 1 unit for 1 mg
1,000 mg10 mL100 mg/mL1 unit for 1 mg

Your NAD+ dose in units

mg
mL
Dose unit
Your syringe
0102030405060708090100

Answer the three questions to see the units to draw, marked on this scale.

Label information

It isn't FDA-approved for any use in people; it's sold as a research chemical. Because no FDA label exists, there are no official mixing instructions; follow what came with the product.

Questions people often ask

How much bacteriostatic water should I use for 500 mg of NAD+?

There isn't one correct amount. With 5 mL, you get 100 mg per mL; adding more water weakens each unit, so small doses are easier to draw.

Can I mix NAD+ with sterile water?

Because sterile water lacks a preservative and is single use, bacteriostatic water is usually used for a mixed vial that you draw from over days. Read about bacteriostatic water compared with sterile water.