How to reconstitute semaglutide
Step-by-step instructions for combining bacteriostatic water with a Semaglutide vial, along with the units in every dose after mixing.
- sizes sold for vials
- 5, 10 mg
- For 5 mg, the water yields 2.5 mg/mL.
- 2 mL
- bac water after the vial is opened
- 28 days
Steps to mix Semaglutide
- After washing your hands, wipe the peptide vial, the bacteriostatic water and each of the two rubber stoppers with an alcohol swab.
- Put the water you chose into the syringe. When you add 2 mL to a 5 mg vial, the result is 2.5 mg per mL.
- At a slight angle, insert the needle through the stopper; let the water flow slowly along the vial's inner wall.
- Until the powder is gone, roll the vial gently in your hands. Don't shake it.
- Mark the vial with the mix date and keep it as your product or prescription directs.
- First, work out the units for your dose; draw to that line afterward.
Mixing a 5 mg vial
When you add 2 mL of water to a 5 mg vial, you get 2.5 mg per mL. A 250 mcg dose comes to 10 units, and a 1 mg dose comes to 40 units. If you use 1 mL instead, each mL contains 5 mg, which makes the same 250 mcg equal to 5 units.
Semaglutide mixing chart
| Vial | Water added | Strength | Units for a sample dose |
|---|---|---|---|
| 5 mg | 1 mL | 5 mg/mL | 5 units for 250 mcg |
| 5 mg | 2 mL | 2.5 mg/mL | 10 units for 250 mcg |
| 5 mg | 3 mL | 1.667 mg/mL | 15 units for 250 mcg |
| 10 mg | 1 mL | 10 mg/mL | 2.5 units for 250 mcg |
| 10 mg | 2 mL | 5 mg/mL | 5 units for 250 mcg |
| 10 mg | 3 mL | 3.333 mg/mL | 7.5 units for 250 mcg |
Unit amounts for your Semaglutide dose
What the label tells you
Ozempic and Wegovy are FDA-approved and come as prefilled pens from Novo Nordisk. Vials that are compounded or used for research are not FDA-approved. The instructions that come with branded pens and vials take precedence over any general steps.
Questions people often ask
What amount of bacteriostatic water does 5 mg of Semaglutide need?
There isn't one correct amount. With 2 mL, you get 2.5 mg per mL; adding more water lowers the amount in each unit, so small doses are easier to draw.
Can I mix Semaglutide with sterile water?
Because sterile water is preservative-free and single use, a vial that you mix and draw from over several days is usually prepared with bacteriostatic water. Read about bacteriostatic water vs sterile water.